Description
Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red
Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Cell Line: | 293 (HEK), 293E (HEK), 293F, Expi293F™, FreeStyle™ 293-F Cells |
|---|---|
| Cell Type: | Kidney (Embryonic) |
| Classification: | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Concentrated: | 1 X |
| Culture Environment: | CO2 |
| Culture Type: | Suspension Cell Culture |
| Endotoxin Level: | < 1 EU/ml |
| Form: | Liquid |
| Format: | Bottle(s) |
| Glucose: | High Glucose |
| Glutamine: | GlutaMAX™-I |
| HEPES Buffer: | No HEPES |
| Included Antibiotics: | No antibiotics |
| Inorganic Salts: | Calcium, Magnesium, Sodium Phosphate |
| Osmolality: | 275-295 mOsm/kg |
| Phenol Red Indicator: | No Phenol Red |
| Product Line: | Expi293™ |
| Product Size: | 1,000 mL |
| Purity or Quality Grade: | Cell Culture Grade |
| Serum Level: | Serum-Free |
| Sodium Bicarbonate Buffer: | Sodium Bicarbonate |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Sterility: | Sterile |
| Supplements: | Dextrose, Insulin-Free, No 2-Mercaptoethanol, No Hypoxanthine, No Thymidine, No Vitamin A, Pyridoxine HCl |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
Contents & storage
Store in the dark at +2°C to +8°C
