List of Components Cat# Description Size EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement 50 ml ;EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250 ml ;EXO-FBSHI-50A-1 Exosome-depleted, Heat Inactivated FBS Media Supplement 50 ml ;EXO-FBSHI-250A- 1 Exosome-depleted, Heat Inactivated FBS Media Supplement 250 ml The bottles of Exo-FBS are sterile and shipped frozen. Thaw the frozen ExoFBS overnight at 4°C to use the next day. Store at 4°C for additional use, do not freeze again. Add the same amount of Exo-FBS as you would standard FBS as a supplement to DMEM (or other media). This is typically 10% in DMEM. Heat inactivated FBS media supplement is treated at 65°C for 15 minutes before bovine exosome removal.